In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:
Note: These cells are distributed subject to the following: 1.） This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.） Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. （603） 646-3675. 3.） In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.
The line was established from cells from liver tissue taken at autopsy of a patient with small cell carcinoma of the lung. The patient had received prior therapy with cytoxan and methotrexate. The cells express HLA class I and class II antigens.
Medium Renewal: Twice per week Remove the spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution （1 to 2 ml） and let the culture sit at room temperature （or 37C） until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Set up flasks at about 9 X 10 exp6 cells per 75 sq. cm. flask.
Preservation:
Culture medium, 95%; DMSO, 5%
References:
22793: Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631
