好看日韩在线视频免费,日本不卡一区二区三区,三级a全过程在线观看,亚洲精品国产9999久久久久

技術(shù)中心

人血小板活化因子(PAF)英文說(shuō)明書(shū)

2013年04月02日 09:17:50人氣:388來(lái)源:上??畦b生物科技有限公司

資料類型jpg文件資料大小3125983
下載次數(shù)89資料圖片 【點(diǎn)擊查看】
上 傳 人上??畦b生物科技有限公司 需要積分0
關(guān) 鍵 詞人血小板,英文說(shuō)明書(shū),(PAF),活化因子
【資料簡(jiǎn)介】

Human PAF

 
FOR RESEARCH USE ONLY
 
Assay range0.6ng/L - 20ng/L                96determinations
Purpose
This kit allows for the determination of PAF concentrations in Humanserum, cellculture supernates and other biological fluids
 
Principle of the assay
The kit assay Human PAFlevel in the sample,use Purified Human PAFantibody to coat microtiter plate wells, make solid-phase antibody, then addPAFto wells,CombinedPAF antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Compley,Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed,reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration ofHuman PAFin the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit

1
wash solution
20ml×1bottle
7
Stopp Solution
6ml×1 bottle
2
HRP-Conjugate reagent
6ml×1 bottle
8
Standard40ng/L
0.5ml×1 bottle
3
Microelisa stripplate
12well×8strips
9
Standard diluent
1.5ml×1bottle
4
Sample diluent
6ml×1 bottle
10
Instruction
1
5
Chromogen Solution A
6ml×1 bottle
11
Closure plate membrane
2
6
Chromogen Solution B
6ml×1 bottle
12
Sealed bags
1

Specimen requirements
1.       extractas soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
2.       Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.       Dilute and add sample:Dilute Original density Standard as follow table:

20ng/L
5 Standard
150μl Original density Standard+150μl Standard diluent
10ng/L
4 Standard
150μl 5 Standard+150μl Standard diluent
5ng/L
3 Standard
150μl 4 Standard+150μl Standard diluent
2.5ng/L
2 Standard
150μl 3 Standard +150μl Standard diluent
1.25ng/L
1 Standard
150μl 2 Standard +150μl Standard diluent

2.add sampleSet blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37.
4.Configurate liquid: 30-fold(or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washingUncover Closure plate membrane, discardLiquid, dry by swing, add washing buffer to every well, still for 30s then drain,repeat 5 times, dry by pat.
6.add enzymeAdd HRP-Conjugate reagent 50μl to each well, except  blank well.
7.incubateOperation with 3.
8.washingOperation with 5.
9.colorAdd Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37
10.Stop the reactionAdd Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assaytake blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Steps description

Standard, Sample diluent

 

AddStandard, Sample diluent, incubate for 30 min at 37.

 

Wash 5 time,AddHRP-Conjugate reagent, incubate for 30 min at 37.

 

Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at 37.

 

AddStopp Solution

 

Read absorbance at 450nm within 15 min

 

calculate

Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Important notes
1.       The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.       washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.       add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
4.       if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluenteand multiplied by the dilution factor.×n×5.
5.       Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.       The substrate evade the light preservation.
7.       Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.       All samples, washing buffer and each kind of reject should according to infective material process.
9.       Do not mix reagents with those from other lots.
 
Storage and validity
1Storage 2-8℃.
2validity six months

上海科鑒生物科技有限公司作者

上一篇:熱收縮帶熱熔膠的選擇

下一篇:熱縮帶的纏繞要求


我要投稿
  • 投稿請(qǐng)發(fā)送郵件至:(郵件標(biāo)題請(qǐng)備注“投稿”)hbzhan@vip.qq.com
  • 聯(lián)系電話0571-87759680
環(huán)保行業(yè)“互聯(lián)網(wǎng)+”服務(wù)平臺(tái)
環(huán)保在線APP

功能豐富 實(shí)時(shí)交流

環(huán)保在線小程序

訂閱獲取更多服務(wù)

微信公眾號(hào)

關(guān)注我們

抖音

環(huán)保在線網(wǎng)

抖音號(hào):hbzhan

打開(kāi)抖音 搜索頁(yè)掃一掃

視頻號(hào)

環(huán)保在線

公眾號(hào):環(huán)保在線

打開(kāi)微信掃碼關(guān)注視頻號(hào)

快手

環(huán)保在線

快手ID:2537047074

打開(kāi)快手 掃一掃關(guān)注
意見(jiàn)反饋
尤物AV无码国产在线看| 大鸡巴射精在小穴动漫版| 亚洲精品伦理熟女国产| 国产区高清在线一区二区三区| 国内精品久久久久精品97| 久久噜噜噜久久熟女精品| 狠狠干无码日韩AV| 国产精品亚洲一区二区三区极品| 97人妻精品一区二区三区视频| 泡芙啪啪啪黄色污污| 国产乱子伦视频一区二区三区| 大几吧插进小穴视频| 被公侵犯中文字幕在线观看| 欧美va精品亚洲va精品| 男人添女人下面免費视頻| 国产妇女乱一性一交| 国产品无码一区二区三区在线| 国产欧美亚洲一区二区三| 久久精品欧美日韩精品不卡| 美女玩奶子和鸡巴| 欧美人与性动交b欧美精品| 久久亚洲精品无码AV宋| 男人草女人的视频免费看| 毛片日产av一区二区三区四区| 国产伦精品一区二区三区视频抖音| 久久久久久久 亚洲精品| 日本精品久久人妻一区二区三区| 大阴茎交于大阴户黄片视频| 猛哥操女人B视频| 大胸美女被c的嗷嗷叫视频| 插欧美美女逼逼逼逼| 色噜噜在线一区二区三区| 91久久愉拍愉拍国产一区| 操俄罗斯美女bb| 亚洲乱码专区一区二区三区四区| 最新的亚洲欧美中文字幕| 大几吧插进小穴视频| 男人插女人视频软件| 亚洲天堂av一区二区在线观看| 大鸡巴插入阴道视频| 精品一区二区三区乱码中文字幕|