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脫氧核糖核酸酶Ⅱ(豬脾)資料說明
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- 【資料簡介】
英文名稱:DNase II ;Deoxyribonuclease Ⅱ(porcine spleen);Deoxyribonucleate 3′-oligonucleotido-hydrolase
其他名稱:去氧細胞核酸酶;氧核糖核酸-5ˊ-寡核苷酸水解酶;無核糖核酸酶;脫氧核糖核酸去聚合酶;脫羥核糖核酸酶(豬胰);除氧細胞核酸酶(豬胰) ;DNA酶
CAS號:9025-64-3
級別:BR
類型:Type IV
活力:2000~6000 units/mg
活力定義:One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25℃; [Mg2+] = 0.83 mM
性狀(以下信息僅供參考):白色或類白色凍干粉。essentially salt-free。為雙鏈特異性內(nèi)切核酸酶,能降解雙鏈DNA成為具有5ˊ-磷酸及3ˊ-OH末端的寡脫氧核糖核苷酸。當有一價錳離子存在時,亦能降解單鏈DNA。溶于水,微溶于30%乙醇和丙酮。酶反應(yīng):脫氧核糖核酸→二核苷酸5′磷酸+寡聚核苷酸5′磷酸zui適pH 7.8。吸光系數(shù)(E1%280)11.1。激活劑為二價金屬離子,zui大激活可以從鎂(Mg2+)+鈣(Ca2+)得到。抑制劑有EDTA和十二烷基硫酸鈉?杉5mmol/L鈣(Ca2+)
用途:本品僅供科研,不得用于其它用途。(以下用途僅供參考)用于分子生物學(xué)研究。
保存:-20℃
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