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【文獻(xiàn)標(biāo)題】Effects of Selenium-Enriched Agaricus blazei Murill on Liver Metabolic Dysfunction in Mice, a Comparison with Selenium-Deficient Agaricus blazei Murill and Sodium Selenite
【作者】Lei Yu ,Shaolong Yang , Lei Sun,et.al
【作者單位】哈爾濱醫(yī)科大學(xué)（Harbin Medical University）
【文獻(xiàn)中引用產(chǎn)品】
小鼠白介素1β (IL-1β)ELISA試劑盒
小鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒
【關(guān)鍵詞】Agaricus blazei Murill . ALT . AST . Alcoholichepatitis . Selenium
【DOI】doi.org/10.1007/s12011-014-0022-8
【影響因子(IF)】1.79
【出版期刊】《Biol Trace Elem Res》
【產(chǎn)品原文引用】
Estimation of Liver IL-1β and TNF-α Level
Liver samples were disintegrated in five volumes of ice-cold RIPA buffer. After incubation on ice for 30 min, samples were centrifuged twice at 20,000×g for 15 min at 4 °C. The resulting supernatants were used for assay. The hepatic concentration of interleukin-1β (IL-1β) and TNF-α was determined using a commercial ELISA kit (Shanghai Jinma Biological Technology, Inc., China) following the manufacture’s instruction.
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