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【文獻標題】Effect of serum from postmenopausal women with osteoporosis exhibiting the Kidney—Yang deficiency pattern on bone formation in an hFOB 1.19 human osteoblastic cell line.
【作者】YACHAN LI1, WENNA LIANG1, XIHAI LI et.al
【作者單位】Fujian University of Traditional Chinese Medicine（福建中醫(yī)藥大學）
【文獻中引用產品】
人雌二醇(E2)ELISA試劑盒
人胰島素樣生長因子結合蛋白1(IGFBP-1)ELISA試劑盒
【關鍵詞】無
【DOI】10.3892/etm.2015.2616
【影響因子(IF)】2.4
【出版期刊】《Experimental and Therapeutic Medicine 》
【產品原文引用】
The serum concentration of estradiol (E2), OPG, and insulin?like growth factor 1 (IGF1) was assessed using ELISA (Shanghai Jinma Biological Technology Co., Ltd, Shanghai, China). All commercial assays were performed according to the manufacturer's instructions.Briefly, ELISA plates were percolated with mouse anti-human immunoglobulin G,and standards, and samples were loaded into the wells and incubated for 1 h at room temperature.HRP—conjugated anti-human E2, OPG and IGF-1 detection antibodies were added and incubated at room temperature for 1 h. The reaction was visualized through color development and the absorbance (optical density) was measured at a 450?nm wavelength on an ELISA reader (Model EXL800; BioTek Instruments, Inc.). The conversion of optical density units for the study samples to concentration was achieved through a computer software-mediated comparison with a standard curve using the KC Junior (BioTek Instruments, Inc.)
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