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產(chǎn)品簡介

CAS號(hào)：產(chǎn)品編號(hào)：A19654 品牌 : CAS號(hào)：別名: ......

詳細(xì)介紹

背景：

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

免疫原信息

免疫原	A synthesized peptide derived from human active + pro Caspase-3.
序列	for sequence
基因ID	836
Swiss Prot	P42574
別名	CPP32;CPP32B;SCA-1;Active Caspase 3;CASP3;active Caspase-3;Caspase 3;Caspase-3 p12;caspase-3

應(yīng)用

反應(yīng)物種	Human, Mouse, Rat
驗(yàn)證應(yīng)用	WBIF
用戶驗(yàn)證的應(yīng)用	WB (Sanghuangporus vaninii , Homo sapiens , Giardia duodenalis , Mus musculus)
推薦稀釋比&實(shí)驗(yàn)步驟	WB 1:500 - 1:2000 [ 實(shí)驗(yàn)步驟 ] IF 1:50 - 1:200 [ 實(shí)驗(yàn)步驟 ]
理論分子量	17kDa/32kDa
實(shí)際分子量	17KDa/32kDa/35KDa
存儲(chǔ)緩沖液	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
實(shí)驗(yàn)方法	Western blotting
陽性樣本	Jurkat,Mouse lung,Mouse liver,NIH/3T3,Rat spleen,Rat liver,293T
細(xì)胞定位	Cytoplasm
純化方式	Affinity purification