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聯(lián)系我時(shí)，請告知來自 環(huán)保在線CAS號(hào)： 產(chǎn)品編號(hào)：A19654 品牌 : CAS號(hào)： 別名: ......
背景：
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
| 免疫原 | A synthesized peptide derived from human active + pro Caspase-3. |
|---|---|
| 序列 | for sequence |
| 基因ID | 836 |
| Swiss Prot | P42574 |
| 別名 | CPP32;CPP32B;SCA-1;Active Caspase 3;CASP3;active Caspase-3;Caspase 3;Caspase-3 p12;caspase-3 |
| 反應(yīng)物種 | Human, Mouse, Rat |
|---|---|
| 驗(yàn)證應(yīng)用 | WBIF |
| 用戶驗(yàn)證的應(yīng)用 | WB (Sanghuangporus vaninii , Homo sapiens , Giardia duodenalis , Mus musculus) |
| 推薦稀釋比&實(shí)驗(yàn)步驟 |
|
| 理論分子量 | 17kDa/32kDa |
| 實(shí)際分子量 | 17KDa/32kDa/35KDa |
| 存儲(chǔ)緩沖液 | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| 實(shí)驗(yàn)方法 | Western blotting |
| 陽性樣本 | Jurkat,Mouse lung,Mouse liver,NIH/3T3,Rat spleen,Rat liver,293T |
| 細(xì)胞定位 | Cytoplasm |
| 純化方式 | Affinity purification |
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