Cyanine5.5 NHS ester,Cy5.5 NHS
Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.
Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.
This reagent can replace NHS esters of Cy5.5®, Alexa Fluor 680, and DyLight 680.
General properties
Appearance: | dark blue powder |
Molecular weight: | 716.31 |
Molecular formula: | C44H46ClN3O4 |
Solubility: | soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water |
Quality contol: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
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Spectral properties
Excitation maximum, nm: | 684 |
ε, L⋅mol−1⋅cm−1: | 209000 |
Emission maximum, nm: | 710 |
Fluorescence quantum yield: | 0.2 |
CF260: | 0.07 |
CF280: | 0.03 |
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