PRP-C18 瑞士 Hamilton 原裝PRP-C18 液相色譜柱
The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any phase or pH. The rigid stationary phase has excellent mechanical and thermal stability(> 100°C), does not shrink or swell, and is compley inert to most conditions commonly encountered in reversed-phase chromatography.
The PRP-C18 is a durable and effective column for general purpose HPLC and especially well-suited for specialized applications in the clinical, pharmaceutical, environmental, food, forensics, and life sciences industries.
PRP-C18 瑞士 Hamilton 原裝PRP-C18 液相色譜柱
色譜柱參數(shù)：
| 固定相 | PRP-C18 |
| 模式 | Reversed-Phase |
| USP 代碼 | USP L21 |
| 填料類型 | PS-DVB functionalized with C18 |
| 填料粒度 | 5 μm |
| 孔徑 | 100 ? |
| 內(nèi)徑 | 4.6 mm |
| 柱管材質(zhì) | 不銹鋼 |
| zui大耐壓 | 5,000 psi |
| Phase Limits | pH 1-13. 0-100%水, 有機改性劑 |
| 緩沖液強度 | 0.0-0.5N |
| 溫度范圍 | 5-85°C |
| 柱子恢復 | Run a gradient of 100% water to 100% acetonitrile. Repeat three times |
| Compound Classification | Organics |
PRP-C18色譜柱-----在任意pH值均可實現(xiàn)高效分離
孔徑: 100A
材料:C18功能化的PS-DVB
在建立方法時,改變流動相的pH值是非常有效的，尤在分離一些中性胺類化合物或者在堿性條件下分離其他的有機堿類化合物。
盡管近年來有宣稱硅膠基C18色譜柱在堿性pH條件下能夠穩(wěn)定工作,但是所有的硅膠基C18色譜柱在pH>6時，測量性能均有所下降,相比在理想條件下使用,色譜柱的使用壽命也顯著縮短。
另一方面，聚合物基質(zhì)色譜柱具有真正的化學穩(wěn)定性,固定相能夠長期耐受濃度高達1M的NaOH和H2SO4 ,且測量性能不會下降。因為在任何pH值下不會出現(xiàn)填料脫落，滲出或溶解現(xiàn)象,所以色譜柱無需考慮流動相狀況在整個使用壽命內(nèi)均可可靠地重復檢測。
高pH值的應用
目前超過70%的藥物成分為陽離子溶質(zhì),在pH<7時,攜帶正電荷,過去一直很難將其與其他有機堿分離。主要是因為離子化在反相色譜法中起決定性作用,電離胺樣品的洗脫窗口十分狹窄;其次帶正電荷的溶質(zhì)和色譜柱固定相上殘余的硅醇基發(fā)生第二次相互作用,常常導致出現(xiàn)峰值形狀不佳,保留時間出現(xiàn)漂移和柱效降低等異?，F(xiàn)象,更進一步縮短色譜柱使用壽命。而PRP-C18可以解決這些問題。
快速洗脫
在現(xiàn)代藥物研發(fā)科學中,常規(guī)分析色譜不應該成為瓶頸。趨勢是使用包含較小顆粒的較短色譜柱并在較高流速下使用,從而提高生產(chǎn)率。PRP-C18目前*要求 。
訂貨信息：
| 訂貨號 | 產(chǎn)品描述 |
| 79477 | Analytical Guard Cartridge Holder, PEEK |
| 32908 | Analytical Guard Cartridge Holder, Stainless Steel |
| 79291 | Analytical Guard Column, PRP-C18 |
| 79641 | Analytical HPLC Column, CUSTOM ORDER (1.0, 2.1, 4.1, 4.6 mm ID) |
| 79643 | Preparative HPLC Column #1, CUSTOM ORDER (21.2, 30 mm ID) |
| 79644 | Preparative HPLC Column #2, CUSTOM ORDER (50 mm ID) |
| 79645 | Preparative HPLC Column #3, CUSTOM ORDER (100 mm ID) |
| 79675 | PRP-C18 5 μm 4.6 x 50 mm |
| 79678 | PRP-C18 12-20 μm 21.2 x 250 mm |
| 79673 | PRP-C18 5 μm 2.1 x 150 mm |
| 79680 | PRP-C18 5 μm 2.1 x 150 mm PEEK |
| 79674 | PRP-C18 5 μm 2.1 x 250 mm |
| 79681 | PRP-C18 5 μm 2.1 x 250 mm PEEK |
| 79672 | PRP-C18 5 μm 2.1 x 50 mm |
| 79679 | PRP-C18 5 μm 2.1 x 50 mm PEEK |
| 79676 | PRP-C18 5 μm 4.6 x 150 mm |
| 79683 | PRP-C18 5 μm 4.6 x 150 mm PEEK |
| 79677 | PRP-C18 5 μm 4.6 x 250 mm |
| 79684 | PRP-C18 5 μm 4.6 x 250 mm PEEK |
| 79682 | PRP-C18 5 μm 4.6 x 50 mm PEEK |
| 79688 | PRP-C18 Analytical Guard Column Replacement Cartridges (5/pk), PEEK |
| 79686 | PRP-C18 Analytical Guard Column Replacement Cartridges (5/pk), Stainless Steel |
| 79687 | PRP-C18 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), PEEK |
| 79685 | PRP-C18 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), Stainless Steel |
| 79690 | PRP-C18 Semiprep/Preparative Guard Column Replacement Cartridges (2/pk), Stainless Steel |
| 79689 | PRP-C18 Semiprep/Preparative Guard Column Starter Kit (1 holder, 1 cartridge), Stainless Steel |
| 79792 | PRP-C18, 10 μm Bulk Resin |
| 79793 | PRP-C18, 12-20 μm Bulk Resin |
| 79791 | PRP-C18, 5 μm Bulk Resin |
| 5095-01 | Semiprep/Preparative Guard Cartridge Holder, Stainless Steel |
| 79918 | Semiprep/Preparative Guard Column, PRP-C18 |
| 79642 | Semipreparative HPLC Column, CUSTOM ORDER (7.0, 7.8, 10 mm ID) |
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