真核生物磷酸鹽轉(zhuǎn)運(yùn)蛋白的晶體結(jié)構(gòu)

研究人員報(bào)告了一個(gè)真菌高親和性磷酸鹽轉(zhuǎn)運(yùn)蛋白PiPT在磷酸鹽存在時(shí)的X-射線晶體結(jié)構(gòu)，它處在一種朝向里面的、被阻隔的狀態(tài)。該結(jié)構(gòu)可以解釋該轉(zhuǎn)運(yùn)蛋白對(duì)于磷酸鹽親和性和結(jié)合特異性的機(jī)制，并將質(zhì)子動(dòng)力與磷酸鹽轉(zhuǎn)位了起來。

PiPT磷酸轉(zhuǎn)運(yùn)蛋白的晶體結(jié)構(gòu)為那些與癌癥和糖尿病等疾病有關(guān)的關(guān)鍵轉(zhuǎn)運(yùn)蛋白等提供了一個(gè)有用模型。

Crystal structure of a eukaryotic phosphate transporter

Bjørn P. Pedersen, Hemant Kumar, Andrew B. Waight, Aaron J. Risenmay, Zygy Roe-Zurz,Bryant H. Chau, Avner Schlessinger, Massimiliano Bonomi, William Harries, Andrej Sali,Atul K. Johri & Robert M. Stroud

Phosphate is crucial for structural and metabolic needs, including nucleotide and lipid synthesis, signalling and chemical energy storage. Proton-coupled transporters of the major facilitator superfamily （MFS） are essential for phosphate uptake in plants and fungi, and also have a function in sensing external phosphate levels as transceptors1, 2, 3, 4, 5. Here we report the 2.9 Å structure of a fungal （Piriformospora indica） high-affinity phosphate transporter, PiPT, in an inward-facing occluded state,