好看日韩在线视频免费,日本不卡一区二区三区,三级a全过程在线观看,亚洲精品国产9999久久久久

技術(shù)中心

CSB-E09501h人甲狀腺結(jié)合球蛋白(TBG)ELISA試劑盒說明書

2011年11月25日 14:22:35人氣:222來源:廈門慧嘉生物科技有限公司

資料類型doc文件資料大小86912
下載次數(shù)96資料圖片 【點擊查看】
上 傳 人廈門慧嘉生物科技有限公司 需要積分0
關(guān) 鍵 詞人甲狀腺結(jié)合球蛋白,TBG ELISA試劑盒,TBG,ELISA試劑盒
【資料簡介】

 

 
 
  
 
 
Human Thyroxine-Binding Globulin(TBG)ELISA Kit     
 
 
 
Catalog No. CSB-E09501h (96 tests)       
l This immunoassay kit allows for the in vitro rapid detection of human TBG concentrations in serum, plasma.
l Expiration date six months from the date of manufacture
l FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
 
廈門慧嘉生物經(jīng)營ELISA試劑盒及抗體、細胞因子、生化試劑、耗材等生物試劑產(chǎn)品。誠信經(jīng)營,價格實惠,服務(wù)周到,質(zhì)量有保證。歡迎廣告老師來詢!:   : /download說明書下載)
 
PRINCIPLE OF THE ASSAY
This assay employs the competitive inhibition enzyme immunoassay technique. A antibody specific to TBG has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated TBG and incubated. A competitive inhibition reaction is launched between TBG (Standards or samples) and Biotin-conjugated TBG with the pre-coated antibody specific for TBG. The more amount of TBG in samples, the less antibody bound by Biotin-conjugated TBG. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of TBG in the sample. The color development is stopped and the intensity of the color is measured.DETECTION RANGE
The standard curve concentrations used for the ELISA’s were 200ng/ml, 100ng/ml, 50ng/ml, 25ng/ml, 12.5ng/ml.
SPECIFICITY This assay recognizes human TBG. No significant cross-reactivity or interference was observed. SENSITIVITY The minimum detectable dose of human TBG is typically less than 6.25ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. MATERIALS PROVIDED

Reagent  
Quantity
Assay plate
1
Standards (S1-S5)
HRP-avidin
1 x 6 ml
Conjugate
1 x 6 ml
Wash Buffer  
1 x 15 ml (20×concentrate)
Substrate A
1 x 7 ml
Substrate B
1 x 7 ml
Stop Solution  
1 x 7 ml

 

Standard
S1
S2
S3
S4
S5
Concentration (ng/ml)
12.5
25
50
100
200

STORAGE
1. Unopened test kits should be stored at 2-8?C upon receipt and the microtiter plate should be kept in a sealed bag with desiccants to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit. Refer to the package label for the expiration date.
2. Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.  
3. A microtiter plate reader with a bandwidth of 10 nm or less and an optical density range of 0-3 OD or greater at 450nm wavelength is acceptable for use in absorbance measurement.
 
TECHNICAL HINTS
1. Bring all reagents and plate to room temperature for at least 30 minutes before use. Unused wells need store at 2-8and avoid sunlight.
2. Wash Buffer  If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have compley dissolved. Dilute 15 ml of Wash Buffer Concentrate into deionized or distilled water to prepare 300 ml of Wash Buffer.
 
 
3. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
4. When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, and/or rotating the plate 180 degrees between wash steps may improve assay precision.
5. Substrate Solution should remain colorless until added to the plate. Keep Substrate Solution protected from light. Substrate Solution should change from colorless to gradations of blue.
6. Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.
 
Precaution: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. OTHER SUPPLIES REQUIRED
? Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
 
 
? Pipettes and pipette tips.
? Deionized or distilled water.
? Squirt bottle, manifold dispenser, or automated microplate washer.
 
SAMPLE COLLECTION AND STORAGE
l Serum Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediay or aliquot and store samples at -20° C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
l Plasma Collect plasma using citrate, EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g within 30 minutes of collection. Assay immediay or aliquot and store samples at -20° C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
 
Note: Grossly hemolyzed samples are not suitable for use in this assay. ASSAY PROCEDURE
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate. All the reagents should be added directly to the liquid level in the well. The pipette should avoid contacting the inner wall of the well.
 
1. Set a Blank well without any solution. Add 50μl of Standard or Sample per well. Standard need test in duplicate. 
2. Add 50μl of Conjugate to each well (not to Blank well), Mix well and then incubate for 1 hour at 37°C. 
3. Fill each well with Wash Buffer (about 200μl), stay for 10 seconds and Spinning. Repeat the process for a total of three washes. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 50μl of HRP-avidin to each well. Incubate for 30mins at 37°C.
5. Repeat the aspiration and wash five times as step 4.
6. Add 50μl of Substrate A and Substrate B to each well, mix well. Incubate for 15 minutes at 37°C. Keeping the plate away from drafts and other temperature fluctuations in the dark.
7. Add 50μl of Stop Solution to each well. 
8. Determine the optical density of each well within 10 minutes, using a microplate reader set to 450 nm.
 
CALCULATION OF RESULTS Average the duplicate readings for each standard, Blank, and sample and subtract the optical density of Blank. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the TBG concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. LIMITATIONS OF THE PROCEDURE
? The kit should not be used beyond the expiration date on the kit label.
? Do not mix or substitute reagents with those from other lots or sources.
 
 
? It is important that the Calibrator Diluent selected for the standard curve be consistent with the samples being assayed.
? If samples generate values higher than the highest standard, dilute the samples with the appropriate Calibrator Diluent and repeat the assay.
? Any variation in Standard Diluent, operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding.
? This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
 
   
PLATE LAYOUT Use this plate layout as a record of standards and samples assayed. 

廈門慧嘉生物科技有限公司作者

上一篇:馬皰疹病毒型PCR檢測試劑盒使用方法

下一篇:人膽囊收縮素八肽ELISA檢測試劑盒使用說明書


我要投稿
  • 投稿請發(fā)送郵件至:(郵件標題請備注“投稿”)hbzhan@vip.qq.com
  • 聯(lián)系電話0571-87759680
環(huán)保行業(yè)“互聯(lián)網(wǎng)+”服務(wù)平臺
環(huán)保在線APP

功能豐富 實時交流

環(huán)保在線小程序

訂閱獲取更多服務(wù)

微信公眾號

關(guān)注我們

抖音

環(huán)保在線網(wǎng)

抖音號:hbzhan

打開抖音 搜索頁掃一掃

視頻號

環(huán)保在線

公眾號:環(huán)保在線

打開微信掃碼關(guān)注視頻號

快手

環(huán)保在線

快手ID:2537047074

打開快手 掃一掃關(guān)注
意見反饋
日韩午夜资源在线观看| 国产蜜月精品高清一区二区三区| 区国产精品搜索视频| 手机成人三级a在线观看| 女生的鸡鸡色色软件| 嗯嗯好硬好大啊老公| 亚洲中文字幕在线无码一区二区| 美女荒郊野外找男人靠逼| 欧美性爱撅臀插入啪啪啪| 欧美精品日韩精品中文字幕| 日韩一区二区三区夜色视频| 亚洲一区二区三成人精品| 高清最新操逼吃鸡巴视频| 日本免费暖暖在线小视频| 亚洲欧美日韩清纯唯美第一区| 最新免费高清无码片| 上萬網友分享a级国产乱| 色熟妇人妻久久中文字幕| 把女生操出水的视频| 大鸡巴干浪穴视频| 操国产骚逼逼逼逼逼逼逼| 鸡巴抽插逼逼视频| 国产品无码一区二区三区在线| 97人人澡人人爽人人揉| 国产一区二区三区免费观在线| 亚洲国产综合精品 在线 一区| 国产黄片在线免费看| 日国产精品1卡二卡三卡| 中文字幕亚洲精品女同一页| 国产午夜精品美女视频露脸| 久久久久精品无码专区喝奶| 差鸡巴没码在线观看| 大屁股真人日逼视频| 男人吃奶大鸡巴操逼视频| 日韩欧美视频在线观看不卡| 亚洲av伦理一区二区三区久久| 精品一区二区三区乱码中文字幕| 国产亂倫近親相姦| 久久精品人人爽人人爽快| 校花内射国产麻豆欧美一区| 亚洲一级片在线播放|