LabAssay? A/G 白蛋白與球蛋白比值檢測試劑盒
【簡單介紹】
《β-葡聚糖 及 β-葡聚糖檢測試劑盒上海甄準生物2013》
β-葡聚糖 073-04811 10 mg ( CAS NO: 9041-22-9) Grade: for Biochemistryβ-Glucan又名β-D-1,3-Glucan 或 Beta-Glucan, Water Soluble
【詳細說明】
β-葡聚糖 及 β-葡聚糖檢測試劑盒
《β-葡聚糖 及 β-葡聚糖檢測試劑盒上海甄準生物2013》
β-葡聚糖 073-04811 10 mg ( CAS NO: 9041-22-9) Grade: for Biochemistryβ-Glucan又名β-D-1,3-Glucan 或 Beta-Glucan, Water Soluble
其他名稱如β-Glucan ;beta-D-Glucan;Beta-1,3-glucan;b-Glucan;
檢測試劑盒如下:
297-51501 SLP Reagent Set微生物(細菌、真菌、酵母)檢測試劑,可用多種測試方法 30-50T
295-53001 SLP Single Reagent Set 微生物(細菌、真菌、酵母)檢測試劑 25T
293-58301 SLP-HS Single Reagent Set 微生物(細菌、真菌、酵母)檢測試劑,高敏感型 20T
β-葡聚糖檢測試劑盒
根據(jù)檢測原理的不同,分為以下幾種
297-51501 SLP Reagent Set(β-D-1,3-Glucan) 3mL (利用家蠶)
或者參考甄準生物,說明書見內。 639-01051 BGSTAR A Kit(beta-1,3-Glucan Assay Kit) (利用鱟)
Amebocyte lysate of horseshoe crabs is highly reactive to endotoxin and has been used as an endotoxin detecting reagent in various fields. The lysate is also reactive to beta-glucan. A purified fraction is prepared when the endotoxin reactive factors and coagulogen are removed from the lysate. The main reagent in this kit is the purified fraction added with the chromogenic synthetic substrate. This reagent is not reactive to endotoxin and specifically detects beta-glucan.
Principle of the test
The factor G in the purified fraction of the lysate reacts with (1->3)-beta-D-glucan. Then the factor G cascade reactions are activated. The clotting enzyme produced in the cascade reactions reacts with the substrate to release p-nitroaniline. The released p-nitroaniline is coupled with the diazocoupling reagent. Then the absorbance of the reaction mixture is measured. The amount of the p-nitroaniline is quantitatively determined.
BGSTAR can be used on a microplate reader by the endpoint method or the kinetic method. In the endpoint method, higher sensitivity can be obtained as the released p-nitroaniline is coupled with the diazo reagent and the absorptivity is enhanced. In the kinetic method, an operator can leave after starting the reaction and the measurable range is wider.
Ordering information
639-01051 BGSTAR A KIT
Contents: (1) Main Reagent, 1 vial x 2.2 mL, (2) Buffer Solution, 1 vial x 3.0 mL, (3) Standard Curdlan, 1 vial x approx. 1,000 pg, (4) beta-Glucan-Free Water, 1 vial x 15 mL, (5) Diazocoupling Reagent, 1 vial x 4.8 mg, (6) Solvent for Diazocoupling Reagent 1, 1 vial x 12 mL, (7) Diazocoupling Reagent 2, 1 vial x 36 mg, (8) Diazocoupling Reagent 3, 1 vial x 8.4 mg
636-01061 BGSTAR B KIT
Contents: (1) Main Reagent, 1 vial x 2.2 mL, (2) Buffer Solution, 1 x 3.0 mL
Note: With one vial of Main Reagent, 40 tests can be performed on a microplate reader. For the kinetic method, Diazocoupling Reagents are not used.
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